Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils

Vanegas David; Abril Novillo Andrea; Khachatryan Aleksandr; Jerves Andrade Lourdes; Peñaherrera Eugenia; Cuzco Nancy; Wilches Isabel; Calle Jessica; Fabián León-Tamariz

doi:10.1186/s13104-021-05838-8

Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils

Vanegas David, Abril Novillo Andrea, Khachatryan Aleksandr, Jerves Andrade Lourdes, Peñaherrera Eugenia, Cuzco Nancy, Wilches Isabel, Calle Jessica, Fabián León-Tamariz

Universidad de Cuenca

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

37 Citas (Scopus)

Resumen

The aim of the present study was to adapt and optimize a broth microdilution method and compare it to the agar dilution method for the evaluation of activity of essential oils from medicinal plants against Gram-negative bacteria. Based on bibliographic research, active and not active oils were selected. The sensitivity and specificity were established as parameters for validation. The comparison between both methods was made using contingency analysis tables, based on the observed frequencies. For both methods, the minimum inhibitory concentration was determined against Escherichia coli strains, in an essential oil concentration range between 0.03 and 0.48% (v/v).

Idioma original	Inglés
Número de artículo	439
Publicación	BMC Research Notes
Volumen	14
N.º	1
DOI	https://doi.org/10.1186/s13104-021-05838-8
Estado	Publicada - dic. 2021

Acceder al documento

10.1186/s13104-021-05838-8

Otros archivos y enlaces

Enlace a la publicación en Scopus

Citar esto

@article{347da3ecdb7f4e43aef29bbe3e836c2b,

title = "Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils",

abstract = "The aim of the present study was to adapt and optimize a broth microdilution method and compare it to the agar dilution method for the evaluation of activity of essential oils from medicinal plants against Gram-negative bacteria. Based on bibliographic research, active and not active oils were selected. The sensitivity and specificity were established as parameters for validation. The comparison between both methods was made using contingency analysis tables, based on the observed frequencies. For both methods, the minimum inhibitory concentration was determined against Escherichia coli strains, in an essential oil concentration range between 0.03 and 0.48\% (v/v).",

keywords = "Escherichiacoli, Essential oils, Microdilution assay, Sensitivity, Specificity, Validation",

author = "Vanegas David and Andrea, \{Abril Novillo\} and Khachatryan Aleksandr and Lourdes, \{Jerves Andrade\} and Pe{\~n}aherrera Eugenia and Cuzco Nancy and Wilches Isabel and Calle Jessica and Fabi{\'a}n Le{\'o}n-Tamariz",

note = "Publisher Copyright: {\textcopyright} 2021, The Author(s).",

year = "2021",

month = dec,

doi = "10.1186/s13104-021-05838-8",

language = "Ingl{\'e}s",

volume = "14",

journal = "BMC Research Notes",

issn = "1756-0500",

number = "1",

}

TY - JOUR

T1 - Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils

AU - David, Vanegas

AU - Andrea, Abril Novillo

AU - Aleksandr, Khachatryan

AU - Lourdes, Jerves Andrade

AU - Eugenia, Peñaherrera

AU - Nancy, Cuzco

AU - Isabel, Wilches

AU - Jessica, Calle

AU - León-Tamariz, Fabián

PY - 2021/12

Y1 - 2021/12

N2 - The aim of the present study was to adapt and optimize a broth microdilution method and compare it to the agar dilution method for the evaluation of activity of essential oils from medicinal plants against Gram-negative bacteria. Based on bibliographic research, active and not active oils were selected. The sensitivity and specificity were established as parameters for validation. The comparison between both methods was made using contingency analysis tables, based on the observed frequencies. For both methods, the minimum inhibitory concentration was determined against Escherichia coli strains, in an essential oil concentration range between 0.03 and 0.48% (v/v).

AB - The aim of the present study was to adapt and optimize a broth microdilution method and compare it to the agar dilution method for the evaluation of activity of essential oils from medicinal plants against Gram-negative bacteria. Based on bibliographic research, active and not active oils were selected. The sensitivity and specificity were established as parameters for validation. The comparison between both methods was made using contingency analysis tables, based on the observed frequencies. For both methods, the minimum inhibitory concentration was determined against Escherichia coli strains, in an essential oil concentration range between 0.03 and 0.48% (v/v).

KW - Escherichiacoli

KW - Essential oils

KW - Microdilution assay

KW - Sensitivity

KW - Specificity

KW - Validation

UR - https://www.scopus.com/pages/publications/85120744264

U2 - 10.1186/s13104-021-05838-8

DO - 10.1186/s13104-021-05838-8

M3 - Artículo

C2 - 34857039

AN - SCOPUS:85120744264

SN - 1756-0500

VL - 14

JO - BMC Research Notes

JF - BMC Research Notes

IS - 1

M1 - 439

ER -

Validation of a method of broth microdilution for the determination of antibacterial activity of essential oils

Resumen

Acceder al documento

Otros archivos y enlaces

Huella

Citar esto