TY - JOUR
T1 - Tanshinone IIA exhibits anticonvulsant activity in zebrafish and mouse seizure models
AU - Buenafe, Olivia Erin
AU - Orellana-Paucar, Adriana
AU - Maes, Jan
AU - Huang, Hao
AU - Ying, Xuhui
AU - De Borggraeve, Wim
AU - Crawford, Alexander D.
AU - Luyten, Walter
AU - Esguerra, Camila V.
AU - De Witte, Peter
PY - 2013/11/20
Y1 - 2013/11/20
N2 - Danshen or Chinese red sage (Salvia miltiorrhiza, Bunge) is used by traditional Chinese medicine (TCM) practitioners to treat neurological, cardiovascular, and cerebrovascular disorders and is included in some TCM formulations to control epileptic seizures. In this study, acetonic crude extracts of danshen inhibited pentylenetetrazol (PTZ)-induced seizure activity in zebrafish larvae. Subsequent zebrafish bioassay-guided fractionation of the extract resulted in the isolation of four major tanshinones, which suppressed PTZ-induced activity to varying degrees. One of the active tanshinones, tanshinone IIA, also reduced c-fos expression in the brains of PTZ-exposed zebrafish larvae. In rodent seizure models, tanshinone IIA showed anticonvulsive activity in the mouse 6-Hz psychomotor seizure test in a biphasic manner and modified seizure thresholds in a complex manner for the mouse i.v. PTZ seizure assay. Interestingly, tanshinone IIA is used as a prescription drug in China to address cerebral ischemia in patients. Here, we provide the first in vivo evidence demonstrating that tanshinone IIA has anticonvulsant properties as well.
AB - Danshen or Chinese red sage (Salvia miltiorrhiza, Bunge) is used by traditional Chinese medicine (TCM) practitioners to treat neurological, cardiovascular, and cerebrovascular disorders and is included in some TCM formulations to control epileptic seizures. In this study, acetonic crude extracts of danshen inhibited pentylenetetrazol (PTZ)-induced seizure activity in zebrafish larvae. Subsequent zebrafish bioassay-guided fractionation of the extract resulted in the isolation of four major tanshinones, which suppressed PTZ-induced activity to varying degrees. One of the active tanshinones, tanshinone IIA, also reduced c-fos expression in the brains of PTZ-exposed zebrafish larvae. In rodent seizure models, tanshinone IIA showed anticonvulsive activity in the mouse 6-Hz psychomotor seizure test in a biphasic manner and modified seizure thresholds in a complex manner for the mouse i.v. PTZ seizure assay. Interestingly, tanshinone IIA is used as a prescription drug in China to address cerebral ischemia in patients. Here, we provide the first in vivo evidence demonstrating that tanshinone IIA has anticonvulsant properties as well.
KW - mouse seizure models
KW - pentylenetetrazol
KW - Salvia miltiorrhiza
KW - Tanshinone IIA
KW - zebrafish PTZ model
UR - http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S2011-21062020000100007
U2 - 10.1021/cn400140e
DO - 10.1021/cn400140e
M3 - Artículo
C2 - 23937066
AN - SCOPUS:84888368707
SN - 1948-7193
VL - 4
SP - 1479
EP - 1487
JO - ACS Chemical Neuroscience
JF - ACS Chemical Neuroscience
IS - 11
ER -