Relationship of anti-müllerian hormone with the quantity and quality of oocytes collected by ultrasound-guided aspiration in Holstein heifers

Jorge Dután; Jorge Samaniego; Fernando Perea; Hugo Hernández-Fonseca; José Luis Pesántez; Andrés Jácome; Gabriela Garay; Luis Ayala

doi:10.52973/rcfcv-e33202

Relationship of anti-müllerian hormone with the quantity and quality of oocytes collected by ultrasound-guided aspiration in Holstein heifers

Título traducido de la contribución: Relación de la hormona anti-mülleriana con la cantidad y calidad de ovocitos colectados por aspiración ecoguiada en vaquillas Holstein

Jorge Dután
, Jorge Samaniego
, Fernando Perea
, Hugo Hernández-Fonseca
, José Luis Pesántez
, Andrés Jácome
, Gabriela Garay
, Luis Ayala

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

1 Cita (Scopus)

Resumen

The experiment had two phases, the first one determined the relationship between the antral follicle count (AFC) and the concentration of Anti-Müllerian hormone (AMH) valued on the day the follicular wave restarted in 61 crossbred Holstein heifers, raised on farms, from the high tropics of Ecuador. The second phase compared the AFC assessed on the day of ultrasound-guided aspiration (OPU); as well as the quantity and quality of Cumulus Oocyte Complexes (COCs) recovered in animals with high (n=8) and low (n=8) concentrations of AMH. Follicular wave synchronization in the first phase of the study was performed with estradiol benzoate plus an intravaginal progesterone (IVD) and prostaglandin (PG) implant. The AFC and the taking of the blood samples in the first phase of the experiment were carried out on day 11 of the protocol. AMH cut-off values of greater than 0.12 ng·mL-1 were established for the high AMH group and 0.05 ng·mL-1 for the low AMH concentration group. The AMH concentration on day 0 (start of the follicular wave) was 0.13 ± 0.01 ng·mL-1, with an AFC of 17.31 ± 0.69 follicles and a high and positive correlation between AMH and the AFC of r=0.93; P<0.001. In the second phase of the experiment, it was observed that the heifers with a high concentration of AMH had double the number of follicles and COCs compared to those with a low concentration; however, the recovery rate was similar (57 %) in the two groups. The existence of a high and positive correlation between AFC and AMH concentration was confirmed; Furthermore, a strong relationship was observed between AMH and the quantity and quality of COCs recovered by OPU. Heifers with greater than 12 ng·mL-1 should be selected as oocyte donors, since they provided double the COC's suitable for the production of embryos in vitro (PIVE), which will influence the quantity and quality of embryos obtained at the end of the PIVE

Título traducido de la contribución	Relación de la hormona anti-mülleriana con la cantidad y calidad de ovocitos colectados por aspiración ecoguiada en vaquillas Holstein
Idioma original	Inglés
Número de artículo	e33202
Publicación	Revista Cientifica de la Facultad de Veterinaria
Volumen	33
DOI	https://doi.org/10.52973/rcfcv-e33202
Estado	Publicada - 2023

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10.52973/rcfcv-e33202

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Enlace a la publicación en Scopus

Rol De La Hormona Antimülleriana Para Predecir La Disponibilidad De Folículos En Vacas, Que Respondan A La Superovulación Y Generen Ovocitos O Embriones Competentes In Vivo E In Vitro
Ayala Guanga, L. E. (Director), Pesantez Pacheco, J. L. (Co-Director), Argudo Garzon, D. E. (Investigador), Narvaez Teran, J. A. (Investigador), Nieto Escandon, P. E. (Investigador), Perea Ganchou, F. P. (Investigador) & Rodas Carpio, E. R. (Investigador)
3/09/18 → 31/08/21
Proyecto: Investigación

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Dután, J., Samaniego, J., Perea, F., Hernández-Fonseca, H., Pesántez, J. L., Jácome, A., Garay, G., & Ayala, L. (2023). Relationship of anti-müllerian hormone with the quantity and quality of oocytes collected by ultrasound-guided aspiration in Holstein heifers. Revista Cientifica de la Facultad de Veterinaria, 33, Artículo e33202. https://doi.org/10.52973/rcfcv-e33202

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title = "Relationship of anti-m{\"u}llerian hormone with the quantity and quality of oocytes collected by ultrasound-guided aspiration in Holstein heifers",

abstract = "The experiment had two phases, the first one determined the relationship between the antral follicle count (AFC) and the concentration of Anti-M{\"u}llerian hormone (AMH) valued on the day the follicular wave restarted in 61 crossbred Holstein heifers, raised on farms, from the high tropics of Ecuador. The second phase compared the AFC assessed on the day of ultrasound-guided aspiration (OPU); as well as the quantity and quality of Cumulus Oocyte Complexes (COCs) recovered in animals with high (n=8) and low (n=8) concentrations of AMH. Follicular wave synchronization in the first phase of the study was performed with estradiol benzoate plus an intravaginal progesterone (IVD) and prostaglandin (PG) implant. The AFC and the taking of the blood samples in the first phase of the experiment were carried out on day 11 of the protocol. AMH cut-off values of greater than 0.12 ng·mL-1 were established for the high AMH group and 0.05 ng·mL-1 for the low AMH concentration group. The AMH concentration on day 0 (start of the follicular wave) was 0.13 ± 0.01 ng·mL-1, with an AFC of 17.31 ± 0.69 follicles and a high and positive correlation between AMH and the AFC of r=0.93; P<0.001. In the second phase of the experiment, it was observed that the heifers with a high concentration of AMH had double the number of follicles and COCs compared to those with a low concentration; however, the recovery rate was similar (57 \%) in the two groups. The existence of a high and positive correlation between AFC and AMH concentration was confirmed; Furthermore, a strong relationship was observed between AMH and the quantity and quality of COCs recovered by OPU. Heifers with greater than 12 ng·mL-1 should be selected as oocyte donors, since they provided double the COC's suitable for the production of embryos in vitro (PIVE), which will influence the quantity and quality of embryos obtained at the end of the PIVE",

keywords = "Anti-m{\"u}llerian hormone, Holstein, follicles, heifers, high tropics",

author = "Jorge Dut{\'a}n and Jorge Samaniego and Fernando Perea and Hugo Hern{\'a}ndez-Fonseca and Pes{\'a}ntez, \{Jos{\'e} Luis\} and Andr{\'e}s J{\'a}come and Gabriela Garay and Luis Ayala",

year = "2023",

doi = "10.52973/rcfcv-e33202",

language = "Ingl{\'e}s",

volume = "33",

journal = "Revista Cientifica de la Facultad de Veterinaria",

issn = "0798-2259",

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TY - JOUR

T1 - Relationship of anti-müllerian hormone with the quantity and quality of oocytes collected by ultrasound-guided aspiration in Holstein heifers

AU - Dután, Jorge

AU - Samaniego, Jorge

AU - Perea, Fernando

AU - Hernández-Fonseca, Hugo

AU - Pesántez, José Luis

AU - Jácome, Andrés

AU - Garay, Gabriela

AU - Ayala, Luis

PY - 2023

Y1 - 2023

N2 - The experiment had two phases, the first one determined the relationship between the antral follicle count (AFC) and the concentration of Anti-Müllerian hormone (AMH) valued on the day the follicular wave restarted in 61 crossbred Holstein heifers, raised on farms, from the high tropics of Ecuador. The second phase compared the AFC assessed on the day of ultrasound-guided aspiration (OPU); as well as the quantity and quality of Cumulus Oocyte Complexes (COCs) recovered in animals with high (n=8) and low (n=8) concentrations of AMH. Follicular wave synchronization in the first phase of the study was performed with estradiol benzoate plus an intravaginal progesterone (IVD) and prostaglandin (PG) implant. The AFC and the taking of the blood samples in the first phase of the experiment were carried out on day 11 of the protocol. AMH cut-off values of greater than 0.12 ng·mL-1 were established for the high AMH group and 0.05 ng·mL-1 for the low AMH concentration group. The AMH concentration on day 0 (start of the follicular wave) was 0.13 ± 0.01 ng·mL-1, with an AFC of 17.31 ± 0.69 follicles and a high and positive correlation between AMH and the AFC of r=0.93; P<0.001. In the second phase of the experiment, it was observed that the heifers with a high concentration of AMH had double the number of follicles and COCs compared to those with a low concentration; however, the recovery rate was similar (57 %) in the two groups. The existence of a high and positive correlation between AFC and AMH concentration was confirmed; Furthermore, a strong relationship was observed between AMH and the quantity and quality of COCs recovered by OPU. Heifers with greater than 12 ng·mL-1 should be selected as oocyte donors, since they provided double the COC's suitable for the production of embryos in vitro (PIVE), which will influence the quantity and quality of embryos obtained at the end of the PIVE

AB - The experiment had two phases, the first one determined the relationship between the antral follicle count (AFC) and the concentration of Anti-Müllerian hormone (AMH) valued on the day the follicular wave restarted in 61 crossbred Holstein heifers, raised on farms, from the high tropics of Ecuador. The second phase compared the AFC assessed on the day of ultrasound-guided aspiration (OPU); as well as the quantity and quality of Cumulus Oocyte Complexes (COCs) recovered in animals with high (n=8) and low (n=8) concentrations of AMH. Follicular wave synchronization in the first phase of the study was performed with estradiol benzoate plus an intravaginal progesterone (IVD) and prostaglandin (PG) implant. The AFC and the taking of the blood samples in the first phase of the experiment were carried out on day 11 of the protocol. AMH cut-off values of greater than 0.12 ng·mL-1 were established for the high AMH group and 0.05 ng·mL-1 for the low AMH concentration group. The AMH concentration on day 0 (start of the follicular wave) was 0.13 ± 0.01 ng·mL-1, with an AFC of 17.31 ± 0.69 follicles and a high and positive correlation between AMH and the AFC of r=0.93; P<0.001. In the second phase of the experiment, it was observed that the heifers with a high concentration of AMH had double the number of follicles and COCs compared to those with a low concentration; however, the recovery rate was similar (57 %) in the two groups. The existence of a high and positive correlation between AFC and AMH concentration was confirmed; Furthermore, a strong relationship was observed between AMH and the quantity and quality of COCs recovered by OPU. Heifers with greater than 12 ng·mL-1 should be selected as oocyte donors, since they provided double the COC's suitable for the production of embryos in vitro (PIVE), which will influence the quantity and quality of embryos obtained at the end of the PIVE

KW - Anti-müllerian hormone

KW - Holstein

KW - follicles

KW - heifers

KW - high tropics

UR - https://www.scopus.com/pages/publications/85172886608

U2 - 10.52973/rcfcv-e33202

DO - 10.52973/rcfcv-e33202

M3 - Artículo

AN - SCOPUS:85172886608

SN - 0798-2259

VL - 33

JO - Revista Cientifica de la Facultad de Veterinaria

JF - Revista Cientifica de la Facultad de Veterinaria

M1 - e33202

ER -

Relationship of anti-müllerian hormone with the quantity and quality of oocytes collected by ultrasound-guided aspiration in Holstein heifers

Resumen

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Rol De La Hormona Antimülleriana Para Predecir La Disponibilidad De Folículos En Vacas, Que Respondan A La Superovulación Y Generen Ovocitos O Embriones Competentes In Vivo E In Vitro

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