Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins

Margarita González-Zamorano; Guillermo Mendoza Hernández; Wendy Xolalpa; Cristina Parada; Antonio J. Vallecillo; Fabiana Bigi; Clara Espitia

doi:10.1021/pr800756a

Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins

Margarita González-Zamorano
, Guillermo Mendoza Hernández
, Wendy Xolalpa
, Cristina Parada
, Antonio J. Vallecillo
, Fabiana Bigi
, Clara Espitia

Universidad Nacional Autónoma de México
Instituto Nacional de Tecnología Agropecuaria

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

100 Citas (Scopus)

Resumen

A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.

Idioma original	Inglés
Páginas (desde-hasta)	721-733
Número de páginas	13
Publicación	Journal of Proteome Research
Volumen	8
N.º	2
DOI	https://doi.org/10.1021/pr800756a
Estado	Publicada - feb. 2009
Publicado de forma externa	Sí

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title = "Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins",

abstract = "A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.",

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AU - González-Zamorano, Margarita

AU - Hernández, Guillermo Mendoza

AU - Xolalpa, Wendy

AU - Parada, Cristina

AU - Vallecillo, Antonio J.

AU - Bigi, Fabiana

AU - Espitia, Clara

PY - 2009/2

Y1 - 2009/2

N2 - A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.

AB - A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.

KW - ConA affinity, Lipoproteins

KW - M. Tuberculosis

KW - Mannosylated

UR - https://www.scopus.com/pages/publications/61849152957

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DO - 10.1021/pr800756a

M3 - Artículo

C2 - 19196185

AN - SCOPUS:61849152957

SN - 1535-3893

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JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 2

ER -

Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins

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