TY - JOUR
T1 - Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins
AU - González-Zamorano, Margarita
AU - Hernández, Guillermo Mendoza
AU - Xolalpa, Wendy
AU - Parada, Cristina
AU - Vallecillo, Antonio J.
AU - Bigi, Fabiana
AU - Espitia, Clara
PY - 2009/2
Y1 - 2009/2
N2 - A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.
AB - A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.
KW - ConA affinity, Lipoproteins
KW - M. Tuberculosis
KW - Mannosylated
UR - https://www.scopus.com/pages/publications/61849152957
U2 - 10.1021/pr800756a
DO - 10.1021/pr800756a
M3 - Artículo
C2 - 19196185
AN - SCOPUS:61849152957
SN - 1535-3893
VL - 8
SP - 721
EP - 733
JO - Journal of Proteome Research
JF - Journal of Proteome Research
IS - 2
ER -
