Glucose and maltose supplementation in the freezing medium of Creole rooster spermatozoa improves in vivo fertility

This study investigated the effects of glucose-maltose supplementation in freezing medium on the kinematic, viability, and fertility of Creole rooster spermatozoa. Fifty ejaculates from 10 Creole roosters were obtained in 5 weekly sessions to create 10 heterologous pools. Each pool was extended with Lake-Ravie plus 6 % dimethylacetamide and allocated to: GM1 (0.64 % glucose + 0.33 % maltose), GM2 (0.72 % glucose + 0.17 % maltose), or non-supplemented control. After thawing, the control presented higher motility and beat-cross frequency than GM1 and GM2 (P < 0.05). Viability was greater in GM1 and control than in GM2 (P < 0.05). Crucially, the fertility after artificial insemination of hens with frozen-thawed sperm from the GM1 group was higher (P < 0.05) compared to the non-supplemented group. In conclusion, glucose-maltose supplementation to the freezing medium can improve the viability and fertility of frozen-thawed Creole rooster semen, supporting its use in genetic conservation and breeding programs.