TY - JOUR
T1 - Efecto Genotóxico del material particulado PM2.5 recolectado en la zona urbana de Cuenca - Ecuador sobre la línea celular de ovario de hámster chino CHO-K1
AU - Vasquez Sigcho, Pamela Elizabeth
AU - Narvaez Vera, Monica Alexandra
AU - Astudillo Aleman, Ana Lucia
AU - Garcia Alvear, Nancy Beatriz
AU - Mejia Coronel, Julio Danilo
AU - Narvaez Vera, Monica Alexandra
AU - Maza, Diego
AU - Moscoso Vanegas, Diana Lucia
AU - Bailon Moscoso, Natalia
PY - 2020/1/1
Y1 - 2020/1/1
N2 - Toxicological and epidemiological studies have a manifesto that particulate matter (PM), specifically PM2.5 has negative effects on human health, associated with mortality, heart failure, respiratory disorders, lung diseases, and cancer. The toxicity and inflammatory effect of these particles are related to their size and chemical characteristics. The objective of this study was to determine the characteristics of the soluble aqueous and organic fractions of the particulate material PM 2.5 collected in the monitoring sites of the urban area of the city of Cuenca-Ecuador and to evaluate its genotoxic activity by means of the test of the comet of in the Chinese hamster ovary cells line CHO-K1. The gravimetric analysis of the samples revealed that two of the study sites exceeded the limit of 25 ug / m3 established by the WHO. In the characterization of water and organic fractions, the presence of metals such as Fe and Zn and aromatic hydrocarbons such as benzo(a) anthracene determined, respectively. Cells were exposed to 3,26 m3 of air /mL of aqueous extracts and 1,63 m3 of air/ mL of organic extracts. Finally, it was observed that the extracts obtained from PM2.5 induce genotoxic in the length of tail length measured by the comet assay; this type of damage can be attributed to the combination of the detected chemical species
AB - Toxicological and epidemiological studies have a manifesto that particulate matter (PM), specifically PM2.5 has negative effects on human health, associated with mortality, heart failure, respiratory disorders, lung diseases, and cancer. The toxicity and inflammatory effect of these particles are related to their size and chemical characteristics. The objective of this study was to determine the characteristics of the soluble aqueous and organic fractions of the particulate material PM 2.5 collected in the monitoring sites of the urban area of the city of Cuenca-Ecuador and to evaluate its genotoxic activity by means of the test of the comet of in the Chinese hamster ovary cells line CHO-K1. The gravimetric analysis of the samples revealed that two of the study sites exceeded the limit of 25 ug / m3 established by the WHO. In the characterization of water and organic fractions, the presence of metals such as Fe and Zn and aromatic hydrocarbons such as benzo(a) anthracene determined, respectively. Cells were exposed to 3,26 m3 of air /mL of aqueous extracts and 1,63 m3 of air/ mL of organic extracts. Finally, it was observed that the extracts obtained from PM2.5 induce genotoxic in the length of tail length measured by the comet assay; this type of damage can be attributed to the combination of the detected chemical species
KW - Material particulado; Genotoxicidad; Ensayo del cometa; Metales pesados; Hidrocarburos aromáticos
KW - Material particulado; Genotoxicidad; Ensayo del cometa; Metales pesados; Hidrocarburos aromáticos
UR - http://rev.aetox.es/wp/wp-content/uploads/2020/04/vol-37.1-14-19.pdf
M3 - Artículo
SN - 0212-7113
JO - Revista de Toxicología
JF - Revista de Toxicología
ER -