TY - JOUR
T1 - BETA ADRENERGIC RECEPTOR ACTIVATION INHIBITS ORAL CANCER MIGRATION AND INVASIVENESS
AU - Bravo-Calderón, Diego Mauricio
AU - Assao, Agnes
AU - Garcia, Natália Galvão
AU - Coutinho-Camillo, Cláudia Malheiros
AU - Roffé, Martin
AU - Germano, Janaína Naiara
AU - Oliveira, Denise Tostes
N1 - Publisher Copyright:
© 2020 Elsevier Ltd
PY - 2020/10
Y1 - 2020/10
N2 - Objective: The aim of this study was to verify β2-AR expression in oral squamous cell carcinoma cell lines (SCC-9 and SCC-25), and to investigate the role of this receptor in migration and invasion of these neoplastic cells. Design: SCC-9 and SCC-25 cells were investigated for gene and protein expression of β2-AR. Cell migration and invasion were analyzed by wound healing assay and transwell invasion camera system. Different concentrations (0.1, 1 and 10 μM) of norepinephrine were used to stimulate, and 1 μM propranolol was used to block the beta-adrenergic receptors on cancer cells. Differences in median values of SCC-9 and SCC-25 and β2-AR protein expression were analyzed by Friedman test and in case of significant differences; pairwise comparisons were performed using Bonferroni correction. Results: The results showed that the β2-AR gene and protein expression were observed in both oral cancer cell lines. The concentration of 10 μM of norepinephrine significantly inhibited (p ≤ 0.05) migration of SCC-9 and SCC-25 cell lines. Furthermore, there was a significant reduction (p ≤ 0.05) in the effect of norepinephrine on cell migration when the β2-AR was inhibited by propranolol. The blockade by propranolol showed a tendency to reverse the effect of norepinephrine on the invasiveness of SCC-9 and SCC-25. Conclusions: The use of beta-adrenergic receptor agonists could become an adjuvant therapeutic target in the treatment of this malignancy.
AB - Objective: The aim of this study was to verify β2-AR expression in oral squamous cell carcinoma cell lines (SCC-9 and SCC-25), and to investigate the role of this receptor in migration and invasion of these neoplastic cells. Design: SCC-9 and SCC-25 cells were investigated for gene and protein expression of β2-AR. Cell migration and invasion were analyzed by wound healing assay and transwell invasion camera system. Different concentrations (0.1, 1 and 10 μM) of norepinephrine were used to stimulate, and 1 μM propranolol was used to block the beta-adrenergic receptors on cancer cells. Differences in median values of SCC-9 and SCC-25 and β2-AR protein expression were analyzed by Friedman test and in case of significant differences; pairwise comparisons were performed using Bonferroni correction. Results: The results showed that the β2-AR gene and protein expression were observed in both oral cancer cell lines. The concentration of 10 μM of norepinephrine significantly inhibited (p ≤ 0.05) migration of SCC-9 and SCC-25 cell lines. Furthermore, there was a significant reduction (p ≤ 0.05) in the effect of norepinephrine on cell migration when the β2-AR was inhibited by propranolol. The blockade by propranolol showed a tendency to reverse the effect of norepinephrine on the invasiveness of SCC-9 and SCC-25. Conclusions: The use of beta-adrenergic receptor agonists could become an adjuvant therapeutic target in the treatment of this malignancy.
KW - beta(2)-adrenergic receptor
KW - Oral cancer
KW - stress
UR - https://www.scopus.com/pages/publications/85089282339
U2 - 10.1016/j.archoralbio.2020.104865
DO - 10.1016/j.archoralbio.2020.104865
M3 - Artículo
C2 - 32801034
AN - SCOPUS:85089282339
SN - 0003-9969
VL - 118
JO - Archives of Oral Biology
JF - Archives of Oral Biology
M1 - 104865
ER -
