Reactivation and Foetal Infection in Pregnant Heifers Infected with Neospora caninum Live Tachyzoites at Prepubertal Age

Yanina P. Hecker; Mercedes M. Burucúa; Franco Fiorani; Jaime E. Maldonado Rivera; Karina M. Cirone; Matías A. Dorsch; Felipe A. Cheuquepán; Lucía M. Campero; Germán J. Cantón; Maia S. Marín; Luis M. Ortega-Mora; Dadín P. Moore

doi:10.3390/vaccines10081175

Reactivation and Foetal Infection in Pregnant Heifers Infected with Neospora caninum Live Tachyzoites at Prepubertal Age

Yanina P. Hecker
, Mercedes M. Burucúa
, Franco Fiorani
, Jaime E. Maldonado Rivera
, Karina M. Cirone
, Matías A. Dorsch
, Felipe A. Cheuquepán
, Lucía M. Campero
, Germán J. Cantón
, Maia S. Marín
, Luis M. Ortega-Mora
, Dadín P. Moore

Consejo Nacional de Investigaciones Científicas y Técnicas
Universidad Nacional de Mar del Plata
Instituto Nacional de Investigación Agropecuaria - Uruguay
Complutense University

Research output: Contribution to journal › Article › peer-review

8 Scopus citations

Abstract

Neospora caninum is recognised for causing cattle abortion, provoking severe economic losses in the livestock industry worldwide. The aim of the present study was to evaluate the reactivation and foetal infection in pregnant heifers inoculated with live N. caninum tachyzoites before puberty. A total of 15 30-month-old pregnant heifers were allocated into four groups: animals inoculated with live tachyzoites of NC-Argentina LP1 isolate before puberty and challenged with live tachyzoites of NC-1 strain at 210 days of gestation (DG) (Group A); animals mock inoculated before puberty and challenged with NC-1 strain at 210 DG (Group B), animals inoculated before puberty but not subsequently challenged (Group C); and noninfected and nonchallenged animals (Group D). The results of this study showed that 100% of animals infected before puberty (Groups A and C) suffered reactivation of the infection at the seventh month of gestation. In addition, in three and two calves from Groups A and C, respectively, congenital infection was confirmed. Interestingly, we provide evidence that the use of live N. caninum tachyzoites in young animals as a strategy to induce protection is neither safe nor effective.

Original language	English
Article number	1175
Journal	Vaccines
Volume	10
Issue number	8
DOIs	https://doi.org/10.3390/vaccines10081175
State	Published - Aug 2022

Keywords

bovine
efficacy
live parasite
Neospora caninum
prepubertal inoculation
reactivation
safety
vertical transmission

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10.3390/vaccines10081175

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Hecker, Y. P., Burucúa, M. M., Fiorani, F., Maldonado Rivera, J. E., Cirone, K. M., Dorsch, M. A., Cheuquepán, F. A., Campero, L. M., Cantón, G. J., Marín, M. S., Ortega-Mora, L. M., & Moore, D. P. (2022). Reactivation and Foetal Infection in Pregnant Heifers Infected with Neospora caninum Live Tachyzoites at Prepubertal Age. Vaccines, 10(8), Article 1175. https://doi.org/10.3390/vaccines10081175

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title = "Reactivation and Foetal Infection in Pregnant Heifers Infected with Neospora caninum Live Tachyzoites at Prepubertal Age",

abstract = "Neospora caninum is recognised for causing cattle abortion, provoking severe economic losses in the livestock industry worldwide. The aim of the present study was to evaluate the reactivation and foetal infection in pregnant heifers inoculated with live N. caninum tachyzoites before puberty. A total of 15 30-month-old pregnant heifers were allocated into four groups: animals inoculated with live tachyzoites of NC-Argentina LP1 isolate before puberty and challenged with live tachyzoites of NC-1 strain at 210 days of gestation (DG) (Group A); animals mock inoculated before puberty and challenged with NC-1 strain at 210 DG (Group B), animals inoculated before puberty but not subsequently challenged (Group C); and noninfected and nonchallenged animals (Group D). The results of this study showed that 100\% of animals infected before puberty (Groups A and C) suffered reactivation of the infection at the seventh month of gestation. In addition, in three and two calves from Groups A and C, respectively, congenital infection was confirmed. Interestingly, we provide evidence that the use of live N. caninum tachyzoites in young animals as a strategy to induce protection is neither safe nor effective.",

keywords = "bovine, efficacy, live parasite, Neospora caninum, prepubertal inoculation, reactivation, safety, vertical transmission",

author = "Hecker, \{Yanina P.\} and Buruc{\'u}a, \{Mercedes M.\} and Franco Fiorani and \{Maldonado Rivera\}, \{Jaime E.\} and Cirone, \{Karina M.\} and Dorsch, \{Mat{\'i}as A.\} and Cheuquep{\'a}n, \{Felipe A.\} and Campero, \{Luc{\'i}a M.\} and Cant{\'o}n, \{Germ{\'a}n J.\} and Mar{\'i}n, \{Maia S.\} and Ortega-Mora, \{Luis M.\} and Moore, \{Dad{\'i}n P.\}",

note = "Publisher Copyright: {\textcopyright} 2022 by the authors.",

year = "2022",

month = aug,

doi = "10.3390/vaccines10081175",

language = "Ingl{\'e}s",

volume = "10",

journal = "Vaccines",

issn = "2076-393X",

number = "8",

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TY - JOUR

T1 - Reactivation and Foetal Infection in Pregnant Heifers Infected with Neospora caninum Live Tachyzoites at Prepubertal Age

AU - Hecker, Yanina P.

AU - Burucúa, Mercedes M.

AU - Fiorani, Franco

AU - Maldonado Rivera, Jaime E.

AU - Cirone, Karina M.

AU - Dorsch, Matías A.

AU - Cheuquepán, Felipe A.

AU - Campero, Lucía M.

AU - Cantón, Germán J.

AU - Marín, Maia S.

AU - Ortega-Mora, Luis M.

AU - Moore, Dadín P.

PY - 2022/8

Y1 - 2022/8

N2 - Neospora caninum is recognised for causing cattle abortion, provoking severe economic losses in the livestock industry worldwide. The aim of the present study was to evaluate the reactivation and foetal infection in pregnant heifers inoculated with live N. caninum tachyzoites before puberty. A total of 15 30-month-old pregnant heifers were allocated into four groups: animals inoculated with live tachyzoites of NC-Argentina LP1 isolate before puberty and challenged with live tachyzoites of NC-1 strain at 210 days of gestation (DG) (Group A); animals mock inoculated before puberty and challenged with NC-1 strain at 210 DG (Group B), animals inoculated before puberty but not subsequently challenged (Group C); and noninfected and nonchallenged animals (Group D). The results of this study showed that 100% of animals infected before puberty (Groups A and C) suffered reactivation of the infection at the seventh month of gestation. In addition, in three and two calves from Groups A and C, respectively, congenital infection was confirmed. Interestingly, we provide evidence that the use of live N. caninum tachyzoites in young animals as a strategy to induce protection is neither safe nor effective.

AB - Neospora caninum is recognised for causing cattle abortion, provoking severe economic losses in the livestock industry worldwide. The aim of the present study was to evaluate the reactivation and foetal infection in pregnant heifers inoculated with live N. caninum tachyzoites before puberty. A total of 15 30-month-old pregnant heifers were allocated into four groups: animals inoculated with live tachyzoites of NC-Argentina LP1 isolate before puberty and challenged with live tachyzoites of NC-1 strain at 210 days of gestation (DG) (Group A); animals mock inoculated before puberty and challenged with NC-1 strain at 210 DG (Group B), animals inoculated before puberty but not subsequently challenged (Group C); and noninfected and nonchallenged animals (Group D). The results of this study showed that 100% of animals infected before puberty (Groups A and C) suffered reactivation of the infection at the seventh month of gestation. In addition, in three and two calves from Groups A and C, respectively, congenital infection was confirmed. Interestingly, we provide evidence that the use of live N. caninum tachyzoites in young animals as a strategy to induce protection is neither safe nor effective.

KW - bovine

KW - efficacy

KW - live parasite

KW - Neospora caninum

KW - prepubertal inoculation

KW - reactivation

KW - safety

KW - vertical transmission

UR - https://www.scopus.com/pages/publications/85137408867

U2 - 10.3390/vaccines10081175

DO - 10.3390/vaccines10081175

M3 - Artículo

AN - SCOPUS:85137408867

SN - 2076-393X

VL - 10

JO - Vaccines

JF - Vaccines

IS - 8

M1 - 1175

ER -

Reactivation and Foetal Infection in Pregnant Heifers Infected with Neospora caninum Live Tachyzoites at Prepubertal Age

Abstract

Keywords

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