Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins

Margarita González-Zamorano; Guillermo Mendoza Hernández; Wendy Xolalpa; Cristina Parada; Antonio J. Vallecillo; Fabiana Bigi; Clara Espitia

doi:10.1021/pr800756a

Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins

Margarita González-Zamorano
, Guillermo Mendoza Hernández
, Wendy Xolalpa
, Cristina Parada
, Antonio J. Vallecillo
, Fabiana Bigi
, Clara Espitia

Universidad Nacional Autónoma de México
Instituto Nacional de Tecnología Agropecuaria

Research output: Contribution to journal › Article › peer-review

100 Scopus citations

Abstract

A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.

Original language	English
Pages (from-to)	721-733
Number of pages	13
Journal	Journal of Proteome Research
Volume	8
Issue number	2
DOIs	https://doi.org/10.1021/pr800756a
State	Published - Feb 2009
Externally published	Yes

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

SDG 3 Good Health and Well-being

Keywords

ConA affinity, Lipoproteins
M. Tuberculosis
Mannosylated

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10.1021/pr800756a

Cite this

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title = "Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins",

abstract = "A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.",

keywords = "ConA affinity, Lipoproteins, M. Tuberculosis, Mannosylated",

author = "Margarita Gonz{\'a}lez-Zamorano and Hern{\'a}ndez, \{Guillermo Mendoza\} and Wendy Xolalpa and Cristina Parada and Vallecillo, \{Antonio J.\} and Fabiana Bigi and Clara Espitia",

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T1 - Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins

AU - González-Zamorano, Margarita

AU - Hernández, Guillermo Mendoza

AU - Xolalpa, Wendy

AU - Parada, Cristina

AU - Vallecillo, Antonio J.

AU - Bigi, Fabiana

AU - Espitia, Clara

PY - 2009/2

Y1 - 2009/2

N2 - A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.

AB - A Mycobacterium tuberculosis culture filtrate enriched with mannose-containing proteins was resolved by 2-DE gel. After ConA ligand blotting, 41 proteins were identified by mass spectrometry as putative glycoproteins with 34 of them new probably mannosylated proteins. These results contribute to the construction of the ConA affinity glycoprotein database of M. tuberculosis, and provide useful information for understanding the biological role of glycoproteins in mycobacteria.

KW - ConA affinity, Lipoproteins

KW - M. Tuberculosis

KW - Mannosylated

UR - https://www.scopus.com/pages/publications/61849152957

U2 - 10.1021/pr800756a

DO - 10.1021/pr800756a

M3 - Artículo

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AN - SCOPUS:61849152957

SN - 1535-3893

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JO - Journal of Proteome Research

JF - Journal of Proteome Research

IS - 2

ER -

Mycobacterium tuberculosis glycoproteomics based on ConA-lectin affinity capture of mannosylated proteins

Abstract

UN SDGs

Keywords

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