TY - JOUR
T1 - Inhibition of Fusarium oxysporum growth in banana by silver nanoparticles
T2 - in vitro and in vivo assays
AU - Mendoza, Natalia Verónica
AU - Yánez Atiencia, Paola Jazmín
AU - Magdama, Freddy
AU - Pacheco, Ricardo
AU - Vielma, Joel
AU - Vanegas Peña, María Eulalia
AU - Bogdanchikova, Nina
AU - Pestryakov, Alexey
AU - Chong, Pablo
N1 - Publisher Copyright:
© 2025 Mendoza et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2025/2/24
Y1 - 2025/2/24
N2 - Fusarium wilt is a devastating disease that affects banana crops worldwide. In Ecuador, bananas are one of the most important commodities and staple food. Nanoparticles are emerging as innovative solutions to control fungal diseases in plant protection. In this study, in vitro and in vivo assays were carried out to validate Fusarium oxysporum growth and disease inhibition. 96-well plates experiments were used to calculate the IC50 of three different silver nanoparticle formulations (Argovit-1220, Argovit-1221, and Argovit-C) against four Ecuadorian Fusarium strains race 1 (EC15-E-GM1, EC19-LR-GM3, EC35-G-GM6, EC40-M-GM2). More than 95% inhibition rate was obtained at 25 mg L−1 concentration. Fusarium wilt in vivo assay (greenhouse conditions) was carried out with Gros Michel plants, where better control was obtained by applying silver nanoparticles to the roots, reducing disease development by an average of 68%. This study shows that silver nanoparticles have a high antifungal potential for controlling the Fusarium wilt of bananas. To our knowledge, this is the first study to test the potential of AgNPs against Fusarium oxysporum race 1 in vitro and in vivo under greenhouse conditions. © 2025 Mendoza et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
AB - Fusarium wilt is a devastating disease that affects banana crops worldwide. In Ecuador, bananas are one of the most important commodities and staple food. Nanoparticles are emerging as innovative solutions to control fungal diseases in plant protection. In this study, in vitro and in vivo assays were carried out to validate Fusarium oxysporum growth and disease inhibition. 96-well plates experiments were used to calculate the IC50 of three different silver nanoparticle formulations (Argovit-1220, Argovit-1221, and Argovit-C) against four Ecuadorian Fusarium strains race 1 (EC15-E-GM1, EC19-LR-GM3, EC35-G-GM6, EC40-M-GM2). More than 95% inhibition rate was obtained at 25 mg L−1 concentration. Fusarium wilt in vivo assay (greenhouse conditions) was carried out with Gros Michel plants, where better control was obtained by applying silver nanoparticles to the roots, reducing disease development by an average of 68%. This study shows that silver nanoparticles have a high antifungal potential for controlling the Fusarium wilt of bananas. To our knowledge, this is the first study to test the potential of AgNPs against Fusarium oxysporum race 1 in vitro and in vivo under greenhouse conditions. © 2025 Mendoza et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
KW - Antifungal agents
KW - Fusarium
KW - Fusarium oxysporum
KW - Metal nanoparticles
KW - Musa
KW - Plant Diseases
KW - Silver
UR - https://www.scopus.com/pages/publications/85219065442
UR - https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0308200
U2 - 10.1371/journal.pone.0308200
DO - 10.1371/journal.pone.0308200
M3 - Artículo
C2 - 39992986
AN - SCOPUS:85219065442
SN - 1932-6203
VL - 20
SP - 1
EP - 15
JO - PLOS One
JF - PLOS One
IS - 2
M1 - e0308200
ER -