Induction of in vitro sprouting of walnut micro-seedlings (Juglans neotropica) treated with Thidiazuron (TDZ) and 6-Benzylaminopurine (BAP)

The walnut (Juglans neotropica) is a species of great potential for conservation projects and eco-agro-production in the Andean region. However, low germination capacity limits its spread for reforestation. The aim of this study was to determine the effect of treatment with Thidiazuron (TDZ) and 6-Benzylaminopurine (BAP) on in vitro shoot induction of J. neotropica. The micro plantlets treated with BAP produced more shoots than those treated with TDZ. The micro plantlets treated with BAP and TDZ in turn generated more shoots than the non-treated micro plantlets. In the test, the effect of concentration of BAP and TDZ in the medium was also evaluated; however not statistically significant differences between the tested concentrations were detected. In summary, findings indicate that the treatment with cytokines, BAP specifically, increases the number of shoots produced by micro plantlets J. neotropica. It is believed that this treatment can be applied on projects of mass propagation of this species within the frame of reforestation programs.