TY - JOUR
T1 - Exploring the virome of Vasconcellea x heilbornii
T2 - the first step towards a sustainable production program for babaco in Ecuador
AU - Cornejo-Franco, Juan F.
AU - Medina-Salguero, Andrés
AU - Flores, Francisco
AU - Chica, Eduardo
AU - Grinstead, Samuel
AU - Mollov, Dimitre
AU - Quito-Avila, Diego F.
N1 - Publisher Copyright:
© 2020, Koninklijke Nederlandse Planteziektenkundige Vereniging.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - The virome of babaco (Vasconcellea x heilbornii) —a non-traditional fruit crop native to Ecuador— was investigated by high-throughput sequencing (HTS) on plants obtained from a commercial nursery. Six virus-like sequences were detected, including the full length of papaya ringspot virus (PRSV) and an RNA-dependent-RNA-polymerase (RdRp) sequence with homology to papaya virus Q. Three RNA sequences were found with homology, respectively, to apple latent spherical virus (genus Cheravirus, 71% nt identity), cherry leaf roll virus (genus Nepovirus, 54% nt identity) and Citrullus lanatus cryptic virus (genus Deltapartitivirus, 66% nt identity); whereas a DNA pararetrovirus-like sequence with homology to citrus endogenous pararetrovirus (58% nt identity) was also detected. RT-PCR-based virus surveys on a total of 284 samples collected from three provinces revealed that the partitivirus- and pararetrovirus-like sequences were present in 100% of tested plants; whereas the other virus sequences were detected in up to 68% of plants and were associated with different symptoms. This work provides information on the occurrence and prevalence of PRSV and five additional virus-like sequences in babaco, a vegetatively propagated crop, supporting the need for a virus-free certification program.
AB - The virome of babaco (Vasconcellea x heilbornii) —a non-traditional fruit crop native to Ecuador— was investigated by high-throughput sequencing (HTS) on plants obtained from a commercial nursery. Six virus-like sequences were detected, including the full length of papaya ringspot virus (PRSV) and an RNA-dependent-RNA-polymerase (RdRp) sequence with homology to papaya virus Q. Three RNA sequences were found with homology, respectively, to apple latent spherical virus (genus Cheravirus, 71% nt identity), cherry leaf roll virus (genus Nepovirus, 54% nt identity) and Citrullus lanatus cryptic virus (genus Deltapartitivirus, 66% nt identity); whereas a DNA pararetrovirus-like sequence with homology to citrus endogenous pararetrovirus (58% nt identity) was also detected. RT-PCR-based virus surveys on a total of 284 samples collected from three provinces revealed that the partitivirus- and pararetrovirus-like sequences were present in 100% of tested plants; whereas the other virus sequences were detected in up to 68% of plants and were associated with different symptoms. This work provides information on the occurrence and prevalence of PRSV and five additional virus-like sequences in babaco, a vegetatively propagated crop, supporting the need for a virus-free certification program.
KW - Babaco
KW - Ecuador
KW - Papaya
KW - Vasconcellea x heilbornii
KW - Virus
UR - https://www.scopus.com/pages/publications/85086655122
U2 - 10.1007/s10658-020-02037-2
DO - 10.1007/s10658-020-02037-2
M3 - Artículo
AN - SCOPUS:85086655122
SN - 0929-1873
VL - 157
SP - 961
EP - 968
JO - European Journal of Plant Pathology
JF - European Journal of Plant Pathology
IS - 4
ER -
