A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives

R. Michael E. Parkhouse; Arturo Carpio; Alfredo Campoverde; Patricia Sastre; Glenda Rojas; Leslie J.S. Harrison; Maria Milagros Cortez

doi:10.1093/trstmh/try116

A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives

R. Michael E. Parkhouse
, Arturo Carpio
, Alfredo Campoverde
, Patricia Sastre
, Glenda Rojas
, Leslie J.S. Harrison
, Maria Milagros Cortez

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Background: Previously we reported the use of a monoclonal antibody-based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods: Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results: The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA. Conclusions: The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.

Original language	English
Pages (from-to)	101-104
Number of pages	4
Journal	Transactions of the Royal Society of Tropical Medicine and Hygiene
Volume	113
Issue number	2
DOIs	https://doi.org/10.1093/trstmh/try116
State	Published - 1 Feb 2019

Keywords

bovine cysticercosis
field diagnosis
HP10 antigen ELISA
HP10 antigen LFA
neurocysticercosis (NCC)

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10.1093/trstmh/try116

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Parkhouse, R. M. E., Carpio, A., Campoverde, A., Sastre, P., Rojas, G., Harrison, L. J. S., & Cortez, M. M. (2019). A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives. Transactions of the Royal Society of Tropical Medicine and Hygiene, 113(2), 101-104. https://doi.org/10.1093/trstmh/try116

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title = "A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives",

abstract = "Background: Previously we reported the use of a monoclonal antibody-based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods: Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results: The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA. Conclusions: The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.",

keywords = "bovine cysticercosis, field diagnosis, HP10 antigen ELISA, HP10 antigen LFA, neurocysticercosis (NCC)",

author = "Parkhouse, \{R. Michael E.\} and Arturo Carpio and Alfredo Campoverde and Patricia Sastre and Glenda Rojas and Harrison, \{Leslie J.S.\} and Cortez, \{Maria Milagros\}",

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doi = "10.1093/trstmh/try116",

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Parkhouse, RME, Carpio, A , Campoverde, A, Sastre, P, Rojas, G, Harrison, LJS & Cortez, MM 2019, 'A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives', Transactions of the Royal Society of Tropical Medicine and Hygiene, vol. 113, no. 2, pp. 101-104. https://doi.org/10.1093/trstmh/try116

A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives. / Parkhouse, R. Michael E.; Carpio, Arturo ; Campoverde, Alfredo et al.
In: Transactions of the Royal Society of Tropical Medicine and Hygiene, Vol. 113, No. 2, 01.02.2019, p. 101-104.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives

AU - Parkhouse, R. Michael E.

AU - Carpio, Arturo

AU - Campoverde, Alfredo

AU - Sastre, Patricia

AU - Rojas, Glenda

AU - Harrison, Leslie J.S.

AU - Cortez, Maria Milagros

N1 - Publisher Copyright: © The Author(s) 2018.

PY - 2019/2/1

Y1 - 2019/2/1

N2 - Background: Previously we reported the use of a monoclonal antibody-based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods: Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results: The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA. Conclusions: The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.

AB - Background: Previously we reported the use of a monoclonal antibody-based (HP10) antigen (Ag) detection lateral flow assay (LFA) for the diagnosis of extraparenchymal neurocysticercosis (EP-NCC). The assay performed well when used with cerebrospinal fluid (CSF) samples but not with their paired serum samples, due to false-positive reactions in some known negative control cases. Methods: Our novel modification involves pretreatment of serum samples using a combination of sodium deoxycholate and dithiothreitol. Results: The modification overcomes the problem of false positives when using negative serum samples from clinically characterized cases of EP-NCC and bovine cysticercosis. In general, there was good agreement between HP10 Ag enzyme-linked immunosorbent assay (ELISA) and the HP10 Ag-LFA, but the HP10 Ag-ELISA was marginally more sensitive than the modified HP10 Ag-LFA. Conclusions: The modified HP10 Ag-LFA provides a field test for the rapid identification of endemic human and bovine cysticercosis.

KW - bovine cysticercosis

KW - field diagnosis

KW - HP10 antigen ELISA

KW - HP10 antigen LFA

KW - neurocysticercosis (NCC)

UR - https://www.scopus.com/pages/publications/85061121773

U2 - 10.1093/trstmh/try116

DO - 10.1093/trstmh/try116

M3 - Artículo

C2 - 30383274

AN - SCOPUS:85061121773

SN - 0035-9203

VL - 113

SP - 101

EP - 104

JO - Transactions of the Royal Society of Tropical Medicine and Hygiene

JF - Transactions of the Royal Society of Tropical Medicine and Hygiene

IS - 2

ER -

A modified lateral flow assay, using serum, for the rapid identification of human and bovine cysticercosis in the absence of false positives

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