Advanced optimization of assisted reproduction in holstein mestizo heifers

Despite the advances in the different reproductive technologies, such as OPU and the PIVE, their implementation in the Bos Taurus dairy genotype is limited. This is because the follicular population (PF) in this genotype is three times less than that in the Bos indicus genotype (19 vs. 36 follicles), this difference in the PF is directly related to intrinsic factors such as the low plasma concentration of gonadotropins such as the stimulating follicle (FSH) and the luteinizing hormone (LH) and with extrinsic factors such as nutrition between Others. To counteract the low plasma concentration of these hormones, the exogenous use of them has been assessed, the first studies have valued the use of the FSH applied intramuscularly with at least eight decreasing doses, this application is due to the fact that this hormone has a short half life of between 2 to 5 hours. To solve this handling, an investigation is intended to be carried out in the farm of the University of Cuenca. Of reproduction in holstein mestizo heifers through advanced technologies, through three experiments. The first will begin with the characterization of the FSH hormonal curve, evaluating different doses and routes of administration (epidural and subcutaneous), with and without adjuvants, to optimize ovarian stimulation, here the hormonal monitoring of blood samples taken in eleven moments will be carried out. In the second experiment, the number and size of antral follicles will be determined postplication of FSH at different doses and routes of administration through follicular ultrasound and aspiration, these oocytes will be classified and after determining their competence will be placed in means of maturation, fertilization and cultivation in in vitro conditions. Finally, in the third experiment, an embryos bank will be established for transfer in extensive livestock in the province of Azuay, in collaboration with small farmers. This bank aims to improve local genetics, evaluating efficiency in terms of pregnancy and embryo transfer, contributing to the sustainable development of extensive systems in the region.

XXI UNIVERSITY COMPETITION FOR RESEARCH PROJECTS