Manithol effects on an experimental model of focal cerebral ischemia

Cerebrovascular disease (ECV) after cancer and coronary heart disease is the most common cause of death throughout the world. In our country in 1999, this was the first cause of mortality. Most ECVs are ischemic type and when they do not cause death they can leave many times invalidating sequelae. Prevention is the best weapon we have against ECVs, and consists in modifying the possible risk factors, but if this is not given and the process of cerebral infarction has already begun, we must quickly act using all the means that are within our reach to minimize neurological damage. Multiple experimental studies are carried out worldwide to establish the most effective therapy that minimizes the damage produced by a cerebral infarction and many are the substances studied. One of them is Manitol, which despite constituting part of the usual treatment scheme for this pathology has not definitely demonstrated its effectiveness in clinical trials. This work is intended to experimentally assess the utility of manitol in the focal cerebral infarction produced in rats. We consider that the use of this substance due to its barred effect of free radicals can decrease the volume of the experimental cerebral infarction in rats, that a dose of 0.25 g/kg./Dose exerts similar effects on the volume of the cerebral infarction to those produced by dose of 1g/kg./Dose and that if the treatment begins within the first 3 hours of initiated the infarction. To do this, we will produce a constant and repeatable model of cerebral infarction in the territory of the middle cerebral artery in 60 laboratory rats, according to the procedures and techniques previously described by other authors and then we will randomly assign them to one of the treatment groups: 1) Control, 2) Manitol at 0.25 g/kg./Dose, 3) Manitol at 1 g/kg. Of initiated the infarction, 5) Start of the treatment at 12 hours after the infarction. The clinical effect of treatments in the different study groups at certain time intervals will be assessed by means of an elementary neurological examination and will finally be evaluated by direct staining and measurement, the volume of the infarcted area in the different cases. Finally, the results obtained in the different groups studied will be compared using the most appropriate statistical tests.

1st UNIVERSITY RESEARCH PROJECT COMPETITION