In vitro determination of the activity of essential oils of medicinal plants and its secondary metabolites against Escherichia coli producer of extended spectrum beta -lactamases

The purpose of the project is to find alternatives for the treatment of infections caused by Escherichia coli producer of extended spectrum beta -lactamases (E.Coli Blee +). It is intended, to take advantage of the biodiversity of the country's southern region to contribute in the search for alternative solutions to increasingly prevalent problems at the hospital and community level, such as infections by microorganisms resistant to antimicrobial choice. Through in vitro techniques, the activity of essential oils of 13 plants from the southern region will be determined, selected based on bibliographic revisions for presenting activity in front of E.Coli strains that are not Blee (+), and whose extracts have been active in previous investigations published by the group of medicinal plants against staphylococcus Aureus ATCC 25923 and to strains of staphyl Resistant meticilino. The proposed research will take place in two years; The team members will contribute in the plants collection phases, obtaining essential oils, microbiological screening, chromatographic analysis, characterization of bioactive metabolites by mass spectrometry, statistical analysis and writing of articles. The scientific contribution will be to standardize a technique for screening of antibacterial activity against gram negative strains and define secondary metabolites responsible for bioactivity with an important social impact by being able to give rational use to effective natural resources to combat strains with a resistance profile and that are frequently causing infections that can range from an infection of the urinary tract to a septicemia.

XVII UNIVERSITY COMPETITION FOR RESEARCH PROJECTS