Comparison of anti -inflammatory activity and chromatographic fingerprint of demodium molliculum and demodium adscendens using in vitro and in vivo models; And identification of the chromatographic peak responsible for the activity and secondary metabolite

Due to the side effects of NSAIDS, of choice for the treatment of inflammatory processes, it is necessary to develop safer therapeutic alternatives. Demodium molliculum is widely used in traditional Ecuadorian medicine for treatment of inflammatory processes; However, it is not endorsed by pharmacological studies. The concentration of plants metabolites is influenced by geographical and environmental conditions, which can be identified by their chromatographic fingerprint. Demodium adscendens and demodium molliculum will be collected from 20 different places, seeking the greatest variability of conditions that allow both characterizing them and differentiating them by their chromatographic profile and anti -inflammatory activity. The extracts obtained by percolation with methanol will be lyophilized prior to their storage and subsequent use. An experimental design will allow selecting the best conditions to ensure adequate separation of peaks in high -resolution liquid chromatography (HPLC) and obtain fingerprints both for D. Molliculum and D. Adscendens. The anti -inflammatory activity of each species will be evaluated in vitro through the inhibition test of hyaluronidase in such a way that the activity differences are evaluated, as well as the differences in composition. When linking anti -inflammatory activity with the fingerprints using a regression model it is intended to obtain the identification of the peaks responsible for said activity, which will be proven in the in vitro model. The anti -inflammatory activity of the extract will be scaled to in vivo model with mice to evaluate its effectiveness in acute and chronic inflammation. The HPPC isolated peak will be analyzed by mass spectrometry to identify the responsible molecule.

XVIII UNIVERSITY COMPETITION FOR RESEARCH PROJECTS