Genotypic characterization of Iberian -type Creole cattle from the province of Azuay

In order to identify and genetically characterize the phenotypically defined Creole livestock population, by determining the DNA sequences of microsatellites defined by the FAO, the Creole bovine populations and pure holstein will be studied, for location, identification and selection of these animals, the information collected in the project of "identification of native breeding races of the native bovine races of the Azuay: Morphometric characterization "that has been executing to date, after the selection of these cattle, a blood sample of between 5 to 10ml will be extracted through the emptying system, the samples obtained will be identified and transported to the Molecular Biology Laboratory of the Faculty of Agricultural Sciences where they will be stored until its processing for obtaining DNA. The extraction and purification of total DNA will be carried out using a commercial kit. Once the DNA is obtained, it will be used for PCR amplification of the 18 genetic markers that will be selected from the 30 suggested by the FAO. Once the PCR products are amplified, they will be taken to a DNA sequencer type 3500 Genetic Analyzers that is located at the facilities of the Faculty of Chemical Sciences of the University of Cuenca. Once the data of each of the alleles of the 18 genetic markers are generated, they will be analyzed through the following genetic tests. To measure genetic diversity, it will be estimated and discussed the values of observed heterozygosis (HO) and expected (He) total and between families (HMO, HME), probability of exclusion (PE), Index of polymorphic content (PIC) and number of alleles by locus. The allelic frequencies will be determined by direct counting, the average expected heterozygosis (He) and observed (HO) values for each of the loci will be obtained using the BIOSS-2 program. For phylogenetic analysis we will use the Neighboor Join (NJ) method

14th RESEARCH PROJECT COMPETITION