Characterization of reproductive biotechnics for conservation of Gametas and Embryos of Creole Bovine, to establish a germplasm bank

The production of in vitro embryos (PIV) is an alternative that in the last 20 years has had a great development in the world and in some countries it has had very large impacts in areas such as: animal production, revenue from germplasm export, cellular cultivation techniques necessary for the development of knowledge and other biotechnologies such as cloning and transgenesis. FAO considers it as a technology of great interest for its application in the conservation of species or individuals. In this sense and as a continuation of a previous project on the "characterization of follicular dynamics and embryo production of the Creole bovine breed compared to two introduced races, Holstein and Brown Swiss", the conservation of germplasm of biotypes Creole bovine adapted to the ecosystem of the province of Azuay will be carried out and that they are in danger of endangered by their absorption with Improved breeds. To do this, the conservation of gametes (semen, oocytes) and embryos produced both in vivo and in vitro obtained respectively from 4 males and 30 females of this biotype will be previously analyzed from the zoosanitary point of view. The general objective of this project will be to form a germplasm bank of Creole cattle in the Azuay region since this biotype is of regional interest, using biotechnics such as the freezing of semen and oocytes (CSyo), the superovulation and in vivo production of embryos (SOV) and with a particular emphasis, through the conservation of embryos produced in vitro (Pive) Methodology towards other possibilities of use. The specific objectives will be: 1-Realize semen extractions of the males to be preserved, evaluate their characteristics and cryopre observe at least 1000 doses of each player; 2-Perform the superovulation and collection of embryos from females that you want to preserve up to a minimum of 5 embryos per female; 3- Complete the facilities and equipment, train professionals and develop the minimum activities to operate a PIVE laboratory; 4- Obtain oocytes by means of follicular puncture and cryopre them; 5- Obtain oocytes by means of follicular puncture, mature and fertilize them in vitro and cultivate them to the blastocyst stadium for later cryopreservation and 6- Achieve sustained production of in vitro embryos with levels greater than 20% with respect to oocytes put in culture. This project then constitutes the confluence of previous actions already carried out in this academic unit to characterize Creole cattle and to produce in vitro embryos.

XIII UNIVERSITY COMPETITION FOR RESEARCH PROJECTS