Molecular biotechnology in the genetic study of rheumatoid arthritis and human virus papilloma. Cuenca 2011-2013

Goals. This research program is aimed at studying two high prevalence health problems that affect the population of the Austro, such as rheumatoid arthritis (AR) and genital infection by human virus papilloma (HPV) responsible for cervical cancer. In the first study, the main objective is the determination of class II histocompatibility antigens in 52 patients with AR and 52 controls and establish the association of these antigens with the rheumatoid factor (FR), anti-CITRULINED ANTIBODS (ANTI-CCP) and rheumatoid nodules. This research will be carried out in patients from the José Carrasco Arteaga (IESS) and Vicente Corral Moscoso (MSP) hospitals. The purpose is to establish the Genotype of Class II HLA of the highest prevalence in patients with rheumatoid arthritis and identify whether these HLA II are expressed with high anti-CCP values. The second study will be carried out in 500 randomized patients in the city of Cuenca, aiming to identify HPV genotypes and cytological characteristics of the cervix, relate them to risk factors for cervical cancer and with existing vaccines in our environment for the prevention of uterine cervical cancer. Utility. The program contributes to the prevention, diagnosis, treatment and monitoring of the ar and cervical cancer; It allows the implementation of new techniques for the biomolecular diagnosis of autoimmune and infectious infectious diseases and allows the sustainability of new scientific research projects related to these issues. Guy. The program has two investigations: determination of the HLA Class II which is a descriptive investigation and virus papilloma, which is a transversal prevalence study. Justification. The program responds to the need to promote scientific research in universities, with a view to solving serious health problems. The research of the HLA Class II and its relationship with laboratory tests and rheumatoid nodules will allow to establish whether the alleles found are different or similar to what is published in Latin America and what type of HLA is associated with severity of the AR with FR and high anti-CCP and rheumatoid nodules; Valuable information to undertake proper treatment. Human viruse's research is aimed at determining the prevalence of papilloma genotypes high and low oncogenic grade in women with active sexual life, correlating the HPV genotype with uterine cervical cancer risk factors, knowing the cytological characteristics of the uterine neck. Uterine neck. Cooperation and complementarity. The program has the cooperation of researchers and strategic partners such as the Vicente Corral Moscoso (MSP) hospital, José Carrasco Arteaga Hospital (IESS), particular Technical University of Loja, Universidad del Rosario de Colombia and University St George's University of London, through their gynecologists, rheumatologists, and researchers and especially users who go to them. Results. These two projects have methodological rigor that guarantee that the results are faithful reflection of reality, and through them we obtain conclusions that are for the benefit of collective health. The results will lead to scientific and technological impact considering the ignorance of reality about the problems under study. Benefits. The beneficiaries of the program will be the patients who have these diseases or are at risk of suffering them, their family and social environment, health institutions, teachers and students of the Faculty of Medical Sciences of the University of Cuenca.

X RESEARCH PROJECT COMPETITION, V RESEARCH PROGRAM COMPETITION.